The key process and information gateway of ischemia/reperfusion injure after improved by drugs and way were researched. Langendorff installation and cardiac muscle of Wistar rats were used. The excitant of adenosine A1 recepor R-PIA was selected as protecting medicine; ATP sensitive potassium channel retarder glybenclamide and PKC inhibitor stauroprine were used simultaneously or separately. The changes of oxygen free radical, nitric oxide, ATPase and inducible nitric oxide synthetase gene were observed, which were compared with ischemia pretreatment at that time. The results showed that R-PIA and ischemia preconditioning had preferable protection and these effects depend on the opening of potassium channel and the activing of PKC. The opening of potassium channel depends on partly the activing of PKC, but opening of potassium channel may be a key factor even more than PKC at downriver position.