To purify the telomerase of HeLa cells and to study its protein components. The method of affinity purifying was used to extract the telomerase component from the crude extract of HeLa cells, basing on the specificity of telomerase containing protein and RNA. And then the activity of telomerase was tested, SDS-PAGE was used to test protein components. There four bands were observed in the SDS-PAGE gel, there are two near 212.2 ku, one near 97.4 ku, one near 42.7 ku compared with the protein mark. It was shown that the product with telomerase activity was obtained using the affinity purification method.