The polymer of the urushiol tyrosine resin coordinating Cu²⁺(UTR-Cu²⁺) was synthesized to immobilize polyphenol oxidases.The results of experiment showed that the method was practicle. Optimum pH of the immobilization polyphenol oxidases was 6.64 and 7.17. The effect of the temperature on UTR-Cu²⁺-enzyme was discussed.Michaelis constant was determined and compared with the free enzyme. It was suggested that the polyphenol oxidases was immobilized by coordination bond, and the model of immobilization enzyme with UTR-Cu²⁺ was proposed.