With the coming of the post-genome era, proteome research, which was sponsored by National Natural Science Foundation of China in our country, has increasingly caught many biochemists’ attention. The fundamental technologies of proteome research are briefly introduced, including sample preparation, protein separation, protein spot detection, gel image analysis, protein identification, database construction, et al. The commonly used identification methods are amino acid analysis, protein sequence tags and peptide mass fingerprinting. The flow chat of proteome analysis is shown, and in particular the latest proteome technologies are summarized.