The earthworm fibrinolytic enzymes (EFE) were separated by affinity chromatography using soybean trypsin inhibitor as a matrix. The enzymes were further separated and purified into 12 components after DEAE-32 chromatography and preparative electrophoresis. The pI of these components gradually decreased from pH 4.0 according to electrophoresis mobility from higher to lower on PAGE. The molecular weights were in the range of 22～34 ku. 6.5 and 7 were glycoproteins proved by staining with the shiff reagent and thymol/sulfuric acid. The fibrinolytic activity of 7 was highest as determined using chromzym UK and chromzym PL as specific substrates.