To find practical and simple transformation methods in transgenic silkworm research, the sperm-mediated methods were tried. The constructed plasmid pFbGFP was transformed into silkworm(Bombyx mori) with three different sperm-mediated transformation methods. Positive results of PCR and Southern blotting was screened in the following two generations. 30% positive rate of the second generation was obtained by one of the methods. This result showed that this was an effective method for transformation of silkworm with foreign gene.