Cloning of AP1 Coactivator JAB1 and Its Association with Hepatopoietin

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Cloning of AP1 Coactivator JAB1 and Its Association with Hepatopoietin
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Fund Project:This work was supported partially by Chinese National Natural Science Foundation Key Project(39830440), Chinese State Key Projects for Basic Research(G1998051122) and Chinese National Natural Science Foundation(30070171).

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Abstract:

Using hepatopioetin (HPO) as “bait” in an yeast two-hybrid screen resulted in the identification of JAB1(a protein initially as a co-activator of c-Jun) as a putative HPO binding partner.Then the full length cDNA of JAB1 was obtained from human fetal liver cDNA library by PCR for GST-JAB1 expression.The binding assay showed that JAB1 binded to rHPO and HPO expressed in COS7 cells in vitro.

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LU Cheng-Rong, LI Yong, DONG Chun-Na, LI Ming, WANG Qing-Ming, ZHANG Cheng-Gang, WEI Yu-Lin, CHEN Hui-Peng, HE Fu-Chu. Cloning of AP1 Coactivator JAB1 and Its Association with Hepatopoietin[J]. Progress in Biochemistry and Biophysics,2001,28(4):528-531

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Received:July 19,2000
Revised:September 28,2000
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