To develop a precise and rapid method for glucose-6-phosphate dehydrogenase (G6PD) assay on the auto-analysis eliminating the interruption of 6-glucose-phosphate dehydrogenase (6GPD).Human blood samples from 107 normal people and 31 patients with obvious G6PD deficiency (Methemoglobin MHb) were analyzed by Rate A with sample blank correction is desired. Results showed that the measured sensitivity was 0.27 U/gHb, the within-run precision was 0.145. the coefficient of variation (CV) was 5.6% and the between-run precision was 0.229. the CV was 9.4%.The linear was between 0～15 U/gHb.Compared to the results measure by Rate A with sample blank correction is desired. The correlation coefficient(r)to two methods was 0.863.These results indicate that it is a sepificity, stable, simple and rapid method and this method is deserved to be popularized.