The expression and subcellular localization of protein kinase C alpha and bata Ⅰ in mouse oocytes were studied by immunofluorochemistry and confocal laser microscopy. Double staining was performed to detect the PKC and cortical granules of the MⅡ stage eggs. The results show that both PKCα and βⅠ were expressed in mouse oocyte at GⅤ and MⅡ stages, but their subcellular localization were different. Simple methods of immunofluorochemistry and confocal laser microscopy in mammalian oocyte were developed. By these methods, the expression and subcellular localization can be studied conveniently and precisely, so as to provide an effective tool to the research of reproductive and developmental biology.