In order to further probe the function of NDR2 gene, the expression of NDR2 gene was determined in different human tissues and their relevant tumors. Tissues of human brain and glioma, lung and lung cancer, colon and carcinoma of colon, stomach and carcinoma of stomach were collected for total RNA extraction and paraffin-embedment. Immunohistochemistry and RT-PCR were used to examine the expression of NDR2 both in mRNA and protein level. DNA sequencing was performed to confirm the result of RT-PCR. Immunohistochemistry results showed that NDR2 protein was extensively expressed in the tissues. RT-PCR further showed an extensive expression of NDR2 mRNA, with a higher expression level in normal brain and lung tissue than glioma and lung cancer respectively, while no differences were observed between colon and carcinoma of colon, stomach and carcinoma of stomach. These results suggest a possible involvement of NDR2 in the genesis and progression of glioma and lung cancer, which provide a clue for further studies on NDR2.