The correlation of glycophorin A and band3 was confirmed by purification and identification of hydrophilic peptides of red blood cell transmembrane domain with high performance liquid chromatograghy(HPLC). Glycophorin A gene(410 bp) was amplified from K562 with RT-PCR and subcloned into the yeast BD hybrid vector pGBKT₇ and baculovirus transfer vector pFASTBac. Glycophorin A and band3 C-terminus were co-transformed into the yeast strain AH109 and the interaction was identified by nutritional selection and β-glycotosidase activity detection. The GPA expression product in Sf9 cells was analyzed on Western blot with anti-GPA and anti-human band3 antibodies, and the result showed that they have immunologic cross-link reaction. All the results above confirmed the interaction between band3 and glycophorin A.