Multi-photon Excitation Imaging of 5-HT Visible Fluorescence in Live Cells

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Multi-photon Excitation Imaging of 5-HT Visible Fluorescence in Live Cells
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Fund Project:This work was supported by grants from Key Program of National Natural Science Foundation of China (59836240), the Youth Fund of National Science Foundation of China (60025514), the National Natural Sciencs Foundation of China (39870205) and Ph.D.Programs

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Abstract:

The autofluorescence of 5-Hydroxytryptamine (5-HT) loaded rat mucosal mast cells (RBL-2H3 cells) was imaged with multi-photon excitation laser scanning microscope (MPELSM). Multi-photon excitated 5-HT relative visible fluorescence is observed in live cells for the first time, and the generating mechanism of 5-HT relative visible fluorescence is preliminarily studied. The spatial distribution of 5-HT in live cells was imaged at high spatial resolution in this present, which provides a new way to study the correlation between 5-HT spatial distribution and content, and the cellular functional state in live tissue or cells.

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ZHANG Zhi-Hong, ZENG Shao-Qun, LUO Qing-Ming, LIU Ya-Feng, ZHOU Wei, CHEN Tong-Sheng. Multi-photon Excitation Imaging of 5-HT Visible Fluorescence in Live Cells[J]. Progress in Biochemistry and Biophysics,2002,29(2):288-292

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Received:July 27,2001
Revised:September 28,2001
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