The cDNA encoding angiogenin was isolated by RT-PCR from human peripheral white blood cell. The bait protein plasmid of pAS-2-1-Ang was constructed, and it's transcription activity was analyzed. Two positive clones were obtained from human fetal liver cDNA library screened by yeast two-hybrid system. The result of sequence analysis and homology comparison showed the candidate protein was lambda-crystallin and human granulin, respectively. The tag plasmid of angiogenin and candidate protein were contructed and cotransfected into COS-7 cell line. The interaction between angiogenin and candidate protein was identified by the assay of co-immunoprecipitation and Western blotting.