With advances in areas such as genome sequencing，robotic，proteomics，microeletronics and bioinformaticd，the field of protein and antibody microarrays has recently had an explosion of interest. Current strategies used to generate protein and antibody arrays, their analytical principle and related detection procedure, as well as their current applications in biological research, medicine and diagnostics are reviesdy. The shortcomings of these approaches, future developments required, and the potential applications of this technique will be discussed.