To confirm whether Epstein-Barr virus latent membrane protein 1 (LMP1) induced telomerase activity of nasopharyngeal epithelial cells through nuclear factor-kappa B(NFκB), NFκB activity analysis was performed with pNFκB-luc reporter plasmid and telomerase activity was tested by PCR-ELISA in primary nasopharyngeal epithelial cells and Tet-on-LMP1 HNE2 cells and other cellular model. The results showed that LMP1 could induce telomerase activity and the C terminus of LMP1 could promote both of NFκB transactivity and telomerase activity. In addition, phosphorothioate olrgonucleotides of antisense NFκB p65 and dominant negative mutant of IκBα could inhibit telomerase activity that was induced by LMP1. It was implicated that LMP1 could regulate telomerase activity via NFκB in nasopharyngeal epithelial cells.