High-throughput Single Nucleotide Polymorphisms Genotyping by Dynamic Allele-specific Hybridization

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High-throughput Single Nucleotide Polymorphisms Genotyping by Dynamic Allele-specific Hybridization
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Fund Project:This work was supported by grants from The Special Funds for Major State Basic Research of China (G1998051008) and The National Natural Sciences Foundation of China (30100027).

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Abstract:

Dynamic allele-specific hybridization (DASH), a new method for rapid, economical, accurate, repeatable and high-throughput genotyping of single nucleotide polymorphisms (SNP), has been used successfully for genotyping 2 SNPs in 96 samples. Experimentation technology has been optimized. Primer design criteria, assay validation and its advantage have also been discussed.

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ZENG Zhao-Yang, XIONG Wei, SHEN Shou-Rong, ZHU Shi-Guo, LI Xiao-Ling, LI Wei-Fang, LI Jiang, ZHOU Ming, FAN Song-Qing, MA Jian, ZHOU Jie, XIAO Bing-Yi, HE Lin, LI Gui-Yuan. High-throughput Single Nucleotide Polymorphisms Genotyping by Dynamic Allele-specific Hybridization[J]. Progress in Biochemistry and Biophysics,2002,29(5):806-810

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Received:March 22,2002
Revised:May 08,2002
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