LIM domain protein KyoT interacts with transcription factor RBP-J and modulates Notch signaling pathway. It was previously found that KyoT was specifically expressed in lung, kidney and testis of mice. To investigate the level of the expression of KyoT in mice embryogenesis and the distribution of the expression the 17 dpc (days of gestation), Northern blot and immunohistochemical SABC methods were used in the experiments. Northern blot showed that KyoT was expressed in almost all stages of embryos of mice and the level was highest in the 17 dpc. Moreover, Northern blot and immunohistochemical SABC showed that the mRNA and protein of KyoT were expressed at the high level in lung, kidney and muscle of 17 dpc mice. These results suggest that KyoT was expressed in mice embryogenesis and the distribution of the expression of KyoT in 17 dpc. was similar to that in adult mice and located in lung, kidney and muscle etc.