RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. In mammal cells, small interfering RNA (siRNA) duplexes can induce RNAi potently, which may provide a new approach to the therapeutics of certain diseases. Focusing on the five genes which coding five crucial proteins of SARS coronavirus(SARS-CoV) respectively, 348 siRNA candidate targets were obtained following bioinformatic methods. In theory, potent siRNA duplexes specifically suppress expression of their corresponding SARS-CoV target gene, while have no influence on the normal expression of human gene. It would lay a foundation for the further experimental researches on the siRNA-like drug design for the SARS-CoV.