In order to develop an alternative and reliable model for further study of human hepatic apolipoprotein CⅢ receptor, and to elucidate the physiological function and regulation of apoCⅢ receptor, whether or not there were apoCⅢ receptors of HepG2 cells by radioligand binding assay was first studied. Addition of increasing concentration of ¹²⁵I-apoCⅢ to HepG2 cells revealed a saturable binding to HepG2 cells with a K_d of （9.53±1.03）×10^-9 mol/L. The maximum specific binding capacity (B_max) was (3.28±0.31) μg/g. The results showed that there were specific, saturable, and reversible binding site of apoCⅢ on HepG2 cells, and it was identified that HepG2 cell line was a useful model for the study of human hepatic apoCⅢ receptor. In the following, the effects of insulin and glucagon on apoCⅢ receptr of HepG2 cells were examined respectively. The results showed that insulin had no effect on the K_d value, but significantly increased the B_max value of apoCⅢ receptor; and glucagon had no effect on the B_max, but decreased the affinity constant of apoCⅢ receptor. These results indicated that human hepatic apoCⅢ receptor was possibliy under the different regulations of insulin and glucagon.