The Direct Observation on The Surface Structure Change of Purple Membrane Induced by High pH Using Atom Force Microscope

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The Direct Observation on The Surface Structure Change of Purple Membrane Induced by High pH Using Atom Force Microscope
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Fund Project:This work was supported by grants from The National Natural Sciences Foundation of China (30170235), The Key Program from The Chinese Academy of Science (KJCX1-SW) and The Special Funds for Major State Basic Research of China (G199801-0102).

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Abstract:

The direct information of the surface structure change of purple membrane under high pH was obtained. The UV-VIS spectra showed that BR was totally denatured and lost its retinal under the pH 12.6. It was found by atom force microscope that the crystalline lattice of purple membrane was broken down at this pH. The typical and non-typical “Island” structures emerged as the BR molecules assemble irregularly in purple membrane.

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Chen De-Liang, Li Hui, Han Bao-Shan, Xu Bing, Hu Kun-Sheng. The Direct Observation on The Surface Structure Change of Purple Membrane Induced by High pH Using Atom Force Microscope[J]. Progress in Biochemistry and Biophysics,2003,30(5):711-714

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Received:July 28,2003
Revised:August 05,2003
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