BRD7 gene is a novel candidate tumor suppression gene associated with NPC. In order to study the function of BRD7, BRD7 was introduced into HNE1 cells by liposome transfection. After staining and image analysis, the ten differential expression spots which up-regulated in BRD7 transfected cells were isolated and identified by two-dimensional polyacrylamide gel eletrophoresis(2D PAGE) and MALDI-TOF. These proteins included argininosuccinate lyase, TSA, proteaseome activator 28 beta subunit, metalloproteinase inhibitor-2 precursor, which involved in cell cycling, transcription regulation,metabolism and so on. The results indicated that the BRD7 gene may play effect on NPC cells by up-regulating the expression of these proteins.