The pIRES2-EGFP eukaryotic expression vectors carrying two kinds of reconstructed human caspase-8 genes with rearrangement subunits were transfected into HeLa cells. The expression and pro-apoptotic effects of reconstructed human caspase-8 gene on HeLa cells were analyzed by indirect fluorescent staining, immunohistochemical staining and electronic microscope etc.The results show that expression of the two human caspase-8 can induce HeLa cells apoptosis effectively.