The baculovirus shuttle vector , pBacPAK-IFN-THY was constructed which contains the genes of IFN-α2b and THY-α1. Constructed vector was coinfected with linear Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened and plaque-purified. The BmN cells were infected with the recombinant virus. The results showed that the protein was successfully prepared and its bioactivities were testified by WISH-VSV system and E-RFC. Results indicated that the expressed fusion protein have bioactivities of both IFN-α2b and THY-α1.