A full-length cDNA amplification technique based on the cap-finder method was used to obtain amplified amount of double strand cDNAs of the endometrial mRNAs at the implantation site from pregnant rhesus monkey. Then a subtractive library of the implantation site was successfully constructed by suppression subtractive hybridization (SSH). Of the randomly selected clones from the library，27% were proved by dot blot analysis to be differentially expressed ones at the implantation site. The data demonstrated that a subtractive library with high quality could be constructed from small amount of rare specimens by the combination of suppression subtractive hybridization（SSH）with full-length cDNA amplification technique based on the cap-finder method.