BLAST analysis suggested that a cDNA fragment (GenBank accession number BG222624) derived from human nasopharynx might represent a novel human gene. Applying the bioinformatics and experimental technique, a novel human gene have been cloned from the fetal brain cDNA library. Since this fragment contained a complete open reading frame(ORF) of 1 254 bp with a stop codon in its upstream and poly(A) signal in its downstream, it could be concluded that it is a full-length gene (GenBank accession number AY174896), which was named as adult retina hypothetical protein(ARHP). The full-length cDNA of ARHP gene is 1 672 bp, coding for a 417 amino acids polypeptide with a predicted molecular mass of 46.58 ku and isoelectric point of 4.20. The deduced amino acid showed 70% homology with a M.musculus protein BAB32214. Bioinformatics analysis suggested that the protein may be a nucleus protein regulating gene transcription. The new gene is comprised of four exons, with three intervening introns and it is localized to chromosome 5q35. It have been found that there are two CpG islands in 5′ UTR of this gene.