In multicellular organisms protein glycosylation is a key post-translational modifications' event. Glycans of glycoproteins are not merely markers to characterize each cell type but are more aggressively involved in numerous biological phenomena, such as cell development, differentiation, implantation, morphogenesis, tumor metastasis and microbe infection. Glycomics is defined to analyse mostly the whole set of glycans of glycoproteins produced in a single organism. Several analysis techniques for separation and identification of glycoproteins and glycans are outlined, and the progress in these techniques is discussed.