ARF can induce cell cycle arrest or apoptosis. By using the A375 cell overexpressing p14^ARF as a model, the molecular machanism of cell cycle arrest by ARF was studied. It was indicated that overexpressing p14^ARF resulted in G1-phase and G2-phase arrest. In the A375 cells overexpressing p14^ARF, the level of p53, p21^cip1 and p27^{kip1 was upregulated, but the protein lever of p-ERK1/2, Cyclin D1 and Cyclin E was inhibited. The results indicated that p14ARF induced the cell cycle arrest of A375 not only in a p53-dependent way, but also in coopration with the ERK signal transduction pathway.}