In order to search for the sequence that affects the entering of PC-1 into cell nucleus，different part of PC-1 cDNA were fused to that of EGFP. Through the observation of the distribution of green fluorescence in the cell，the region between 112～190 amino acids was identified as independent functional domain which excluded PC-1 from cell nucleus and there appeared many dotted structure in the cell cytoplasm. The other part of PC-1 could accumulated in the nucleus when this region was deleted. Simultaneously，PC-1 was also found to attach to the cell membrane by the yeast two-hybrid system based on the SOS recovery sytem，what is more，the region between 112～190 amino acids was identified as independent domain responsible for it is cell membrane anchoring.