The results of many researches show that 3′untranslated region plays a very important role in the life cycle of single strand plus RNA virus. The high structures in 3′UTR such as stem-loop, pseudoknot and tRNA-like structure are also generally believed to be partly involved into the transcription, replication and translation of plus-strand RNA virus. The common methods and the last results about 3′UTR of this kind of virus are reviewed.