Tubulin is one superfamily of proteins. α- and β-tubulins are the major proteins of microtubules, while γ-tubulin plays a critical role in the nucleation of microtubules. Western blotting and immunofluorescent confocal microscopy were used to study the assembly and transformation of γ-tubulin during meiotic maturation, fertilization and parthenogenetic activation of pig oocytes. γ-Tubulin exists in the porcine oocytes and its quantity remains stable during meiotic maturation. It is located in the region where there is microtubule assembly, especially on the spindle poles at metaphase and middle plate or midbody of elongating spindle at anaphase and telophase. After in vitro fertilization and parthenogenetic activation, γ-tubulin concentrated around the male and female chromatin and pronuclei. It is also found to be localized in sperm acrosomal cap and the neck. During early cleavage, γ-tubulin distributes around the blastomere nuclei. The results suggest that γ-tubulin is a major regulator of microtubule assembly in porcine eggs and that both oocyte and sperm contribute centrosome materials to fertilized eggs.