Effort was made to screen expressed genes of a white-rot fungus Trametes gallica by using cDNA microarray from Phanerochaete chrysosporium, a well known model strain used for studying lignin-degrading systems. The results showed that there were only 172 positive clones detected on the microarray and 95.9% of the clones had a ratio of Cy-5/Cy-3 from 0.5 to 2.0. However, only 3 and 4 clones with the Cy-5/Cy-3 ratio over 2.0 and lower than 0.5 respectively, were found, which are corresponding to 5- and 12-day cultures of T.gallica. One hundred and twenty-two clones were randomly selected and sequenced, of which 118 clones could be perfectly located in the genome of P.chrysosporium. The result indicates that there is obvious sequence difference between probes from T.gallica and inserts on the chip from P.chrysosporium, exhibiting far relative between these two fungi. Two interesting clones have been obtained from homology comparison, one corresponding to a fragment of peroxidase lpoB gene of P.chrysosporium and the other encoding a sort of heat shock protein.