In order to study the interactions between plant virus movement protein and pectin methylesterase (PME), PME gene from tobacco genome was cloned using RT-PCR method and the sequence was determined (GenBank accession No.AY238968). Sequence analysis showed that there were two conserved domains in PME protein: PMEI and pectinesterase. Multiple copies of PME gene were detected in tobacco genome through Southern blot analysis. Western blot result indicated that two types of PME protein existed in tobacco. But Northern hybridization detected only a full length transcript of PME, which further identified that there was post-translational process. Yeast two-hybrid result demonstrated that PME could not interact with the identified movement protein of rice dwarf virus (RDV), Pns6, but does interact with Pns11, a nucleic acid binding protein of RDV. It implies Pns11 may participate in the movement of RDV.