The process of in vivo patch clamp is to recoding electrophysiological signal of neurons in vivo by whole cell techniques, which is useful approach in physical and pharmacological research. A blind patch-clamp technique in the intact brain is usually adopted. Now a new visual method are reported and termed as two-photon targeted patching, which uses two-photon imaging to guide in vivo whole-cell recordings to individual, genetically labeled cortical neurons, so that subset of neurons are specially focused. Here both two methods were simply reviewed.