The gene encoding the cytoplasmic domain of human Fas from 175aa to 304aa was amplified from U937,which was then determined by sequence analysis and cloned into pC4M-FV2E. The resulting Myr-FKBP12-Fas fusion gene was subcloned into pEGFP-N1.Stable cell clones as named 293/ FKBP12-Fas were obtained by transfecting the recombinant plasmid pEGFP-N1/FKBP12-Fas into HEK293,which was following selected by G418. The integration and expression of FKBP12-Fas fusion gene were identified by RT-PCR and Western-blotting respectively. Both the observation of the condensation and margination of the chomatin on nuclear membrane under electron microscopy and the detection of typical “DNA Ladder” proved the apoptosis induced by AP20187. However, FK506 could competitively block such cell apoptosis, indicating that the inducible apoptosis cell model established as above offered an applicable technical platform for the screening of FK506-like small molecular compounds.