A Gateway-compatible Agrobacterium sp. binary vector system for high-throughput functional analysis of genes in plants has been produced, but a bottleneck problem using this system for fast and reliable DNA cloning is how to obtain entry clones for the PCR products or other DNA fragments simply, economically and efficiently. To address this problem, the traditional TA cloning and the Gateway recombinant cloning techniques are integrated, and two kinds of TA cloning vectors compatible with the Gateway cloning are constructed. The vectors can be used for cloning PCR products or other DNA fragments and at the same time making entry clones in a simple, economical and efficient way.