BRD7, a novel bromodomain gene, has been cloned by cDNA RDA (cDNA Representational Difference Analysis). The GenBank accession number is AF152604. Previous studies showed that BRD7 gene was down-regulated in nasopharyngeal carcinoma (NPC) cells and tissues. Over expression of BRD7 in NPC cells can inhibit cell proliferation and cell cycle progression from G1 to S phase, and can partly inhibit the aberrant growth of NPC cells. In order to explore the molecular mechanisms that involved in the down-regulation of BRD7 gene in NPC cells, bioinformatic approaches were adopted and a putative promoter region was found. The luciferase expression vectors containing BRD7 promoter region were further constructed . Transient transfection results suggested that the analyzed sequence contained BRD7 promoter. Transcriptional factor Sp1 is responsive to this region. Inhibition of the Sp1 binding to BRD7 promoter by mithramycin A significantly reduced the promoter activity and the endogenous expression of BRD7 in mRNA level.