The defects and dysfunctions in antigen-presenting dendritic cells have been shown during carcinogenesis leading to human immune system can be markedly suppressed by cancer. To explore the mechanisms by which cancers escape from immune recognition, the influences of tumor microenvironment on differentiation of dendritic cells (DCs), which play an important role in tumor immunology, were studied by biophysical and microrheological methods. It was found that the components derived from tumors caused the increases in osmotic fragility and decreases in membrane fluidity of DCs, moreover, the gene transcriptional levels and energy statuses of cells were also transformed, leading to impaired capabilities of antigen uptaking and deteriorated stimulatory capacities to activate na?觙ve T cells. The impaired microrheological properties of DCs may be one of the aspects of immune escape mechanism of tumor.