Staphylococcus epidermidis is the common pathogen of nosocomial infection. SarA (staphylococcal accessory regulator A) is a global factor regulating expression of most virulence genes in S. epidermidis. It is reported that SarA regulates the transcriptions of lipA encoding lipase, zinC encoding membrane- associated zinc metalloprotease and atlE encoding autolysin. SarA exhibiting an up-regulation on lipA and zinC, and down-regulation on atlE were demonstrated by reverse-transcription PCR and reporter gene lacZ analysis respectively. The results demonstrate that SarA regulated directly the expressions of lipA, zinC and atlE by protein-DNA binding to the promoter region of AT-rich sequence. The consensus sequence of binding sites in atlE, lipA and zinC promoter regions of S.epidermidis RP62A was forecasted by using program Omiga 2.0. The results suggest that SarA could be treated as the target in the prevention of Staphylococcus epidermidis infection.