With Saccharomyces cerevisiae cell surface engineering, combining with enzyme-linked immunosorbent assay (ELISA), a new method has been, which is named as ‘yeast-ELISA’ detection. Here, the recombination yeast cell MT8-1 surface display expressing antigen HPSα6 (Human proteasome subunit alpha 6), and its monoclonal antibody 3D7D12D4 (hybridoma cell line No) was used as study object. The fitful yeast cell concentration of this method is 0.50 A₆₀₀, and it was used to detect monoclonal antibody and its valance (1∶5×10⁵). The specificity and sensitivity of the method was demonstrated by crossing test and interdiction test. The results indicated that ‘yeast-ELISA’ could detect monoclonal antibody directly, and the valance of which is concordance with the result of the proteantigen indirect ELISA. And also this method could be used to antiserum detection, which also has good result. It demonstrates that the method of the ‘yeast-ELISA’ detection was established, which is the supply and application of Saccharomyces cerevisiae cell surface engineering.