Preparation of Monoclonal Antibody Against “a” Determinant With Mutation on Site 145 and Rapid Diagnosis Assay for HBV Mutant Strain

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Preparation of Monoclonal Antibody Against “a” Determinant With Mutation on Site 145 and Rapid Diagnosis Assay for HBV Mutant Strain
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Fund Project:This work was supported by a grant from The Science and Technology Development Planning Project of Jilin Province (20060923-02), and The Bureau of Science and Technology of Changchun City (2005-120/05GG76).

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Abstract:

Hepatitis B virus surface antigen with mutation on site 145 may cause omission in detection of hepatitis B virus with HBsAg diagnostic reagent. Monoclonal antibody against this mutation was obtained through hybridoma technique. The sub-class of the monoclonal antibody was determined to be IgG1(κ type, the titer is 1∶9×10⁴), and the monoclonal antibody has no cross-reaction to other hepatitis virus. The method of AC-ELISA which was established with monoclonal antibody and polyclonal antibody showed high analysing susceptibility for HBV mutant samples.

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WANG Jun, WANG Ji, WANG Li. Preparation of Monoclonal Antibody Against “a” Determinant With Mutation on Site 145 and Rapid Diagnosis Assay for HBV Mutant Strain[J]. Progress in Biochemistry and Biophysics,2008,35(2):231-234

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Received:June 12,2007
Revised:June 29,2007
Accepted:
Online: November 08,2007
Published: February 20,2008

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