To study the regulation mechanism of SOCS-3 (Suppressor of cytokines signals-3) for erythropoietic development, small interference RNA expression vectors of SOCS-3 were constructed and transferred in to the K562 cell lines stably by lentiviral system. The efficiency of virus transfection was identified by expression of green fluorescence protein(GFP) analyzed by fluorescence microscope, then the high GFP expression K562 cells were sorted by fluorescence-activated cell sorting (FACS) according to strong GFP expression. The efficiency of RNA interferencing on SOCS-3 were detected by Real time-PCR and Western blot. The SOCS-3 gene expression at mRNA level of K562 cells transfected by lentivirus plasmids was 22.1% of the K562 cells transfected by the control lentivirus. The SOCS-3 protein expression of K562 cells transfected by lentivirus plasmids was also down regulated. Furthermore, K562 cells transfected by lentivirus plasmids were induced into the erythropoietic cells and the erythropoietic differentiation of K562 cells were examined by benzidine staining, immunocytochemistry and RT-PCR. Then it was found that the K562 cells could be induced into erythroid lineage cells more easily after silencing of SOCS-3. The experiment confirmed the important role of SOCS-3 in erythropoietic development and provided a useful new way for production of erythroid lineage cells.