To explore whether the 3′-untranslated region (3′-UTR) of human high-affinity sodium-dependent dicarboxylate transporter (hSDCT2) plays a role in the regulation of gene expression, sequence characteristics of the 3′-UTR was analyzed using bioinformatics. The results found that within the 3′-UTR of SDCT2β mRNA there is an AU-rich region (AUR) of 585 nt, which contains three AU-rich elements (ARE). The AUR fragment was inserted into the 3′-UTR of GFP reporter gene within a expression vector pcDNA-GFP; and a pcDNA-GFP-AUR recombinant vector was constructed and transfected into HEK293, HKC and LLC-PK1 cell lines. The expression level of intracellular GFP was determined by Western blot and flow cytometry. The results showed that the AUR of SDCT2β could significantly reduce the expression level of GFP in the pcDNA-GFP-AUR- transfected cells (P < 0.01). After blockade of RNA transcription with actinomycin D, total RNA was extracted from stably transfected HEK293 cells with an interval of 2 h, and the stability of GFP-AUR and GFP mRNAs was analyzed by Northern blot. The results indicated that the stability of GFP-AUR mRNA is less than that of GFP mRNA. These results suggest that within the AU-rich region of 3′-UTR of SDCT2β mRNA there is a negative regulation region which can decrease the stability of mRNA, accelerate the degradation of GFP mRNA and may play a negative regulation role for gene expression at post-transcriptional level.