To investigate the mRNA expression profile of novel candidate of tumor suppressor gene NGX6 in the several common types of cancer, analyze the correlation between NGX6 mRNA expression and its clinicopathological and to evaluate the validity that NGX6 mRNA act as molecular marker for tumor metastasis and prognosis. Multi-tumor tissue and nasopharyngeal cancer tissue microarrays were constructed previously and tissue microarrays combined with in situ hybridization were used to detect the expression of NGX6 mRNA in the human several common types of cancer. Results showed that expression of NGX6 mRNA in the nasopharyngeal cancer (NPC), lung cancer, gastric cancer and colon-rectal cancer was significantly lower than that in their non-cancer normal tissue (P < 0.05, P < 0.01). Expression level of NGX6 mRNA was evidently lower in the NPC, laryngeal cancer, lung caner and colon-rectal cancer with lymph node metastasis (P < 0.05, P < 0.01). Expression of NGX6 mRNA in the NPC, lung caner and colon-rectal cancer had significant correlation with their clinical stages, which NGX6 mRNA was significantly lower in the clinical stage T2, T3 or T4 that than their clinical stage T1(P < 0.05, P < 0.01). The results suggested that the NPC, lung caner, gastric cancer and colon-rectal cancer had evidently lower expression of NGX6 mRNA, NGX6 mRNA might be used as molecular markers for invasion, metastasis and prognosis of NPC, lung caner and colon-rectal cancer.