FAAP (focal adhesion associated protein), encoded by murine D10Wsu52e gene, is a member of function-unknown protein family, UPF0027. These protein family homologues are conserved and ubiquitously expressed in various species, organs and cells. But the functions are quite unclear yet. Here, FAAP were demonstrated to deposited in the cytoplasmic fractions and the plasma membrane fractions by ultra-speed centrifugation. The expression levels of FAAP are significantly correlative with 67 ku Laminin receptor (LR) in HeLa cells. Moreover, FAAP, similar with LR, could effectively restrain the cultured cells attachment. Together, these results suggest the effects of FAAP on cell attachment.