The ripeness of tomato is regulated by multi-genes. Transcripts of mRNA extracted from tomato at breaker stage were normalized, and cDNA with pTRV was constructed after reverse transcription. In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing. PDS, which was related about synthesis of lycopene, was used as marker gene to establish the model of screening. Among one hundred mixed agrobateria, PDS was successfully screened in the first stage of model establishment.