TAK1 and TAK2 are both nuclear-encoded kinases. TAK1 may be involved in the phosphorylation of LHCⅡ in state transitions. After the analysis of conservative activity regions on TAK1 and TAK2 sequences, a segment of hydrophilic polypeptide consisting of 12 amino acid residues was devised and linked to bovine serum albumin (BSA) before injection to the rabbit. The polyclonal antibody against TAK1 raised was examined by agar gel immunodiffusion (AGID) test and Western blot analysis. then it was used to study the expression of tak1 and tak2 influenced by the change of light and temperature in RT-PCR with specific primers and the possible regulation relationship between TAK1 and LHCⅡ phosphorylation especially by using TAK1 serum and P-Thr antibody. The result indicated that light and temperature regulated the phosphorylation of LHCⅡ and took effect on the transcription and translation level of tak1 and tak2, but the response of LHCⅡ phosphorylation to light did not coincided with the quantity of TAK1 protein. Furthermore, low light density could enhance the expression of tak2, but temperature impacted tak2 little. The different regulation pattern of tak1 and tak2 may derive from the different elements of promoter.