To investigate action mechanism of the expression of rVBMDMP gene regulated by hTERT promotor inhibited cell growth of lung cancer, the Core domain of hTERT promotor was obtained by PCR and its function activity was detected. Then, retroviral vector of pLNSX/hTERT/rVBMDMP was recombined, and lung cancer A549 cells were infected by the collected vetrovirus. Further, it was observed that expression of rVBMDMP gene regulated by hTERT promotor influenced cellular morphous, cell growth, cell apoptosis and expression of Caspase-3 protein. Moreover, it was observated that expression of rVBMDMP gene regulated by hTERT promotor inhibited the growth of transplanted tumor in the nude mice, the cellular apoptosis of tumor tissue and the expression of Caspase-3 protein. The results showed: a. The Core domain of hTERT promotor can regulate the expression of rVBMDMP gene (n=3, P < 0.05); b. Expression of rVBMDMP gene regulated by hTERT promotor was abled to inhibit the growth of lung cancer A549 cells and transplanted tumor in nude mice (n=6, P < 0.05); c. The expression of rVBMDMP gene promoted the apoptosis of lung cancer A549 cells and the expression of Caspase-3 protein. These results suggested that expression of rVBMDMP gene regulated by hTERT promotor inhibited the growth of lung cancer A549 cells because it enhanced expression level of Caspase-3 protein to increase cell apoptosis.