The theory of topological visual perception was proposed in previous studies. According to this theory, topological properties, as a global properties, is the primitive of visual perception, and figures with topological differences could be discriminated easier than figures with local feature differences. The different topological properties pattern recognition was investigated in mice by using a modified Y-maze. Two stimuli of topological different features were used to train mice to distinguish between ring and square. Besides, a ring and a square, the other stimulus which topologically features different or same carried as test. These stimulus patterns include a hollow square, a disk, a ring with a gap and a hollow square with a gap. The results showed that the mice trained to discriminate the ring (the reward pattern) from the square(the not reward pattern)were unable to distinguish between the disk and the square as well as the ring and the hollow square, but were able to discriminate the hollow square from the ring with a gap, the hollow square with a gap or the disk, respectively. The data suggest that visual system in mice may be sensitive to topological distinction and extract the topological properties to transfer. Moreover, these findings provide a new evidence for the hypothesis that topological perception is a fundamental property of vision.