To dissect the splicing mechanism is a critical step in understanding the plant morphogenesis, growth, development and responses to stresses. Compared to animals, the research on RNA splicing is progressing poorly in plants. The splicing patterns of gene fragments derived from monocot rice BADH2 and dicot Arabidopsis GR7 were detected by Agrobacterium-mediated tobacco transient expression system. The results indicated that the fundamental splicing regulatory elements are conserved in plants. The tobacco transient expression system could be used as an important tool for fast and sensitive detection of gene splicing regulation in higher plants.