Aging process is modulated by various genes and signaling pathways. Mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase (S6K) not only regulate the physiological functions of cells, but also play an important role in the ageing progression. Recent study showed that inhibiting mTORC1 or S6K can extend the life span of mice. mTOR signaling influences ageing through various ways, including cell autophagy, metabolic by-products accumulation and affects the number of stem cells in tissues. However, the role of S6K in the ageing process remains unclear yet. Studies on the role of these two molecules in the ageing process, which have become a hotspot, are expected to find the method to extend the life span of mammalian and reveal its mechanisms. This review will focus on the role of mTOR and S6K in ageing.